AIM:
Using a standardized splenic injury model of uncontrolled hemorrhagic shock, we aimed to research the effect of different fluid resuscitation on the liver arginase activity.
MATERIALS AND METHODS:
Fifty rats were divided into five groups. In group 1; massive splenic injury was untreated, in group 2 massive injury was treated with 70 ml/kg/hour Ringer Lactate solution, in groups 3,4,5 massive injury was treated with 7.5 ml/kg/hour Hydroxyethyl Starch, Hypertonic Saline and Dextran-40, respectively between 15 and 30 minutes of injury. In all groups, splenectomy was performed at the 30th minute. Hemodynamic monitoring and anesthesia were continued up to 90 minutes. Duration of follow-up was 48 hrs. After 48 hours of survival, liver tissue samples were removed and were kept in -70◦C for the analysis of arginase activities.
RESULTS:
Different fluid regimens were found to have no effects on hemodynamic parameters, arteriel blood gases and survival at the early phase of uncontrolled hemorrhagic shock. RL increased liver arginase activity compared with control group (p<0.02) and other groups. (p<0.05). Unlike Ringer Lactate, other groups had no statistical differences at arginase activities comparing to control group.
CONCLUSIONS:
Finally, depending on long term results, Ringer Lactate is more useful fluid choice in hemorrhagic shock.
Keywords: Hemorrhagic shock, splenic injury, Ringer lactate, arginase activity